A TRANSPARENT AGAR MEDIUM FOR GROWING NEISSERIA GONORRHOEAE

Agar dilution method for susceptibility testing of Neisseria gonorrhoeae.

The antibiotic susceptibilities of Neisseria gonorrhoeae isolates obtained from patients attending a clinic for sexually transmitted diseases in Tucumán, Argentina, were determined by the agar dilution method (MIC). 3.5% of the isolates produced beta-lactamase. A total of 96.5% of beta-lactamase negative isolates tested were susceptible to penicillin (MIC < or = 2 micrograms/ml-1); 14.03% of th...

Agar Gradient Diffusion Susceptibility Testing for Neisseria gonorrhoeae: A Reliable Alternative to Agar Dilution?

A defined agar medium for genetic transformation of Neisseria meningitidis.

Catlin, B. Wesley (Marquette University, Milwaukee, Wis.) and Gertrude M. Schloer. A defined agar medium for genetic transformation of Neisseria meningitidis. J. Bacteriol. 83:470-474. 1962.-An agar medium was developed for use in quantitative genetic studies of Neisseria meningitidis strain 15. It contains eight inorganic salts, sodium citrate, sodium lactate, arginine, cysteine, glycine, sodi...

Further studies with a selective medium for cultivating Neisseria gonorrhoeae.

A culture medium that is selective for the gonococcus and the meningococcus has been described recently (Thayer and Martin, 1964). The medium with Polymyxin and Ristocetin added permits isolation of the gonococcus from sites highly contaminated with other bacteria. Overgrowth of gonococcal colonies by the commensal bacterial flora present in rectal, vaginal, and cervical specimens is almost com...

Carbon dioxide requirement of Neisseria gonorrhoeae growing on a solid medium.

A survey of 120 isolations of Neisseria gonorrhoeae from male patients showed that 47 (39%) isolates were unable to produce visible colonies without a supply of exogenous carbon dioxide. Of 63 strains, 25 strains required CO2 for isolation but none exhibited a CO2 requirement beyond subculture 4. CO2-requiring strains deprived of CO2 appeared to lose their colony-forming ability exponentially i...